Postdoc position: Mass Spectrometry of Epigenetic DNA/RNA Modifications 1118 views

The Institute of Molecular Biology funded by the Boehringer Ingelheim Foundation is inviting applications for a Postdoc position in Mass Spectrometry of Epigenetic DNA/RNA Modifications (#CNPD11).

About the project:

• The ERC-funded laboratory of Christof Niehrs studies regulatory DNA and RNA modifications, which play important roles in development & disease.
• The lab utilizes highly sensitive mass spectrometry (Triple Quadrupole Mass Spec) and stable isotope metabolic labelling to profile epigenetic and epitranscriptomic changes, including 5mC, 5hmC, 5fC, 5caC, m6A, and others.
• The successful candidate will study nucleic acid modifying enzymes to unravel how chemical modifications in DNA, RNA and R-loops regulate pluripotency and differentiation of mammalian embryonic stem cells and embryos.
• The selected candidate will use mass spectrometry, as well as genome-edited CRISPR/Cas9 stem cell mutants, and also various NGS protocols to carry out a multi- dimensional analysis to address: Which modifications can be identified and how do they change with different cellular states? Where in the genome, and in which RNAs, do the modifications occur? What are molecular determinants involved in targeting of modifications to specific sites?
• The project will involve strong interaction with computational biologists.

Starting Date: preferably by 15.01.2020

Duration: Initially 2 years; option for further extension

Requirements:

• PhD & publications in a relevant field
• Experience in mass spectrometry and RNA/DNA biochemistry
• Excellent communication skills and good team spirit
• Ability to solve problems independently
• Fluent in English (spoken and written)

Applications:

Apply by sending a SINGLE PDF file containing cover letter stating research and career interests, CV, scans of degrees (Germans: including copies of Abitur- & Master degree), and contact details of 2 references to personnel@imb-mainz.de (quoting job reference #CNPD11).